文章基本信息

标题：A Role of the Putidaredoxin COOH-terminus in P-450cam (Cytochrome m) Hydroxylations
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作者：S. G. Sligar ; P. G. Debrunner ; J. D. Lipscomb 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：10
页码：3906-3910
DOI：10.1073/pnas.71.10.3906
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Methylene hydroxylation by cytochrome P-450cam (cytochrome m) can be resolved into four distinct steps: substrate addition, mo [->] mos; reduction, mos [->] mrs; dioxygen addition, mrs [->] mO2rs; followed by a second putidaredoxin (Pseudomonas putida ferredoxin)-mediated reduction and product formation. The isolated ferrous oxy-substrate complex exhibits first-order decay kinetics with the relatively slow rate constant of k [unk] 0.01 sec-1, at 25{degrees
关键词：monooxygenase activity ; protein modification ; iron-sulfur protein-cytochrome complex ; fluorescence ; cytochrome P -450