文章基本信息

标题：The Nucleotide Sequence of the lac Operator
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作者：Walter Gilbert ; Allan Maxam
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：12
页码：3581-3584
DOI：10.1073/pnas.70.12.3581
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The lac repressor protects the lac operator against digestion with deoxyribonuclease. The protected fragment is double-stranded and about 27 base-pairs long. We determined the sequence of RNA transcription copies of this fragment and present a sequence for 24 base pairs. It is: 5'--T G G A A T T G T G A G C G G A T A A C A A T T 3' 3'--A C C T T A A C A C T C G C C T A T T G T T A A 5' The sequence has 2-fold symmetry regions; the two longest are separated by one turn of the DNA double helix.
关键词：regulation ; protein-nucleic acid interaction ; DNA-RNA sequencing ; oligonucleotide priming