文章基本信息

标题：Requirement of a Phage-Induced 5′-Exonuclease for the Expression of Late Genes of Bacteriophage T5
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作者：G. Chinnadurai ; D. J. McCorquodale
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：12
页码：3502-3505
DOI：10.1073/pnas.70.12.3502
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Amber mutants of bacteriophage T5 defective in gene D15, which codes for a 5'-exonuclease, do not express late genes. Electrophoretic separation in sodium dodecyl sulfate-polyacrylamide gels of the proteins induced by this mutant in nonpermissive Escherichia coli show a virtual absence of late proteins. Synthesis of lysozyme and serum-blocking power is very low whereas the extent of synthesis of an early enzyme, deoxyribonucleoside monophosphokinase, is similar to that in wild-type infections. It is proposed that one requirement for the expression of late T5 genes is the introduction of gaps or nicks in the T5 DNA so that late transcription can occur.
关键词：electrophoresis ; transcription ; “late protein” synthesis