文章基本信息

标题：Conformational selection and induced fit mechanism underlie specificity in noncovalent interactions with ubiquitin
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作者：Tomasz Wlodarski ; Bojan Zagrovic
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2009
卷号：106
期号：46
页码：19346-19351
DOI：10.1073/pnas.0906966106
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Noncovalent binding interactions between proteins are the central physicochemical phenomenon underlying biological signaling and functional control on the molecular level. Here, we perform an extensive structural analysis of a large set of bound and unbound ubiquitin conformers and study the level of residual induced fit after conformational selection in the binding process. We show that the region surrounding the binding site in ubiquitin undergoes conformational changes that are significantly more pronounced compared with the whole molecule on average. We demonstrate that these induced-fit structural adjustments are comparable in magnitude to conformational selection. Our final model of ubiquitin binding blends conformational selection with the subsequent induced fit and provides a quantitative measure of their respective contributions.
关键词：ubiquitin binding ; protein recognition ; Kolmogorov–Smirnov test