文章基本信息

标题：Mouse prickle1, the homolog of a PCP gene, is essential for epiblast apical-basal polarity
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作者：Hirotaka Tao ; Makoto Suzuki ; Hiroshi Kiyonari 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2009
卷号：106
期号：34
页码：14426-14431
DOI：10.1073/pnas.0901332106
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Planar cell polarity (PCP) genes are essential for establishing planar cell polarity in both invertebrate and vertebrate tissues and are known to regulate cellular morphogenesis and cell movements during development. We focused on Prickle, one of the core components of the PCP pathway, and deleted one of two mouse prickle homologous genes, mpk1. We found that the deletion of mpk1 gene resulted in early embryonic lethality, between embryonic day (E)5.5 and E6.5, associated with failure of distal visceral endoderm migration and primitive streak formation. The mpk1-/- epiblast tissue was disorganized, and analyses at the cellular level revealed abnormal cell shapes, mislocalized extracellular matrix (ECM) proteins, and disrupted orientation of mitotic spindles, from which loss of apico-basal (AB) polarity of epiblast cells are suspected. Furthermore, we show mpk1 genetically interacts with another core PCP gene Vangl2/stbm in the epiblast formation, suggesting that PCP components are commonly required for the establishment and/or the maintenance of epiblast AB polarity. This was further supported by our finding that overexpression of {Delta}PET/LIM ({Delta}P/L), a dominant-negative Pk construct, in Xenopus embryo disrupted uniform localization of an apical marker PKC{zeta
关键词：anterior visceral endoderm ; primitive streak ; Vangl2/stbm