文章基本信息

标题：Translation and replication of hepatitis C virus genomic RNA depends on ancient cellular proteins that control mRNA fates
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作者：Nicoletta Scheller ; Leonardo Bruno Mina ; Rui Pedro Galão 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2009
卷号：106
期号：32
页码：13517-13522
DOI：10.1073/pnas.0906413106
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Inevitably, viruses depend on host factors for their multiplication. Here, we show that hepatitis C virus (HCV) RNA translation and replication depends on Rck/p54, LSm1, and PatL1, which regulate the fate of cellular mRNAs from translation to degradation in the 5'-3'-deadenylation-dependent mRNA decay pathway. The requirement of these proteins for efficient HCV RNA translation was linked to the 5' and 3' untranslated regions (UTRs) of the viral genome. Furthermore, LSm1-7 complexes specifically interacted with essential cis-acting HCV RNA elements located in the UTRs. These results bridge HCV life cycle requirements and highly conserved host proteins of cellular mRNA decay. The previously described role of these proteins in the replication of 2 other positive-strand RNA viruses, the plant brome mosaic virus and the bacteriophage Q{beta
关键词：deadenylation-dependent mRNA decay ; HCV ; host factors ; LSm1–7 ; Rck/p54