文章基本信息

标题：Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector
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作者：Federico Gonzalez ; Montserrat Barragan Monasterio ; Gustavo Tiscornia 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2009
卷号：106
期号：22
页码：8918-8922
DOI：10.1073/pnas.0901471106
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Induced pluripotent stem (iPS) cells have generated keen interest due to their potential use in regenerative medicine. They have been obtained from various cell types of both mice and humans by exogenous delivery of different combinations of Oct4, Sox2, Klf4, c-Myc, Nanog, and Lin28. The delivery of these transcription factors has mostly entailed the use of integrating viral vectors (retroviruses or lentiviruses), carrying the risk of both insertional mutagenesis and oncogenesis due to misexpression of these exogenous factors. Therefore, obtaining iPS cells that do not carry integrated transgene sequences is an important prerequisite for their eventual therapeutic use. Here we report the generation of iPS cell lines from mouse embryonic fibroblasts with no evidence of integration of the reprogramming vector in their genome, achieved by nucleofection of a polycistronic construct coexpressing Oct4, Sox2, Klf4, and c-Myc.
关键词：iPS cells ; nonintegrative technique ; reprogramming ; pluripotency