文章基本信息

标题：Single-nucleotide discrimination in immobilized DNA oligonucleotides with a biological nanopore
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作者：David Stoddart ; Andrew J. Heron ; Ellina Mikhailova 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2009
卷号：106
期号：19
页码：7702-7707
DOI：10.1073/pnas.0901054106
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The sequencing of individual DNA strands with nanopores is under investigation as a rapid, low-cost platform in which bases are identified in order as the DNA strand is transported through a pore under an electrical potential. Although the preparation of solid-state nanopores is improving, biological nanopores, such as {alpha}-hemolysin ({alpha}HL), are advantageous because they can be precisely manipulated by genetic modification. Here, we show that the transmembrane {beta}-barrel of an engineered {alpha}HL pore contains 3 recognition sites that can be used to identify all 4 DNA bases in an immobilized single-stranded DNA molecule, whether they are located in an otherwise homopolymeric DNA strand or in a heteropolymeric strand. The additional steps required to enable nanopore DNA sequencing are outlined.
关键词：α-hemolysin ; DNA sequencing ; genomics ; protein engineering ; protein pore