文章基本信息

标题：High-resolution functional proteomics by active-site peptide profiling
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作者：Eric S. Okerberg ; Jiangyue Wu ; Baohong Zhang 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2005
卷号：102
期号：14
页码：4996-5001
DOI：10.1073/pnas.0501205102
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Characterization and functional annotation of the large number of proteins predicted from genome sequencing projects poses a major scientific challenge. Whereas several proteomics techniques have been developed to quantify the abundance of proteins, these methods provide little information regarding protein function. Here, we present a gel-free platform that permits ultrasensitive, quantitative, and high-resolution analyses of protein activities in proteomes, including highly problematic samples such as undiluted plasma. We demonstrate the value of this platform for the discovery of both disease-related enzyme activities and specific inhibitors that target these proteins.
关键词：capillary electrophoresis ; fluorescence ; MS ; protease