文章基本信息

标题：Structural origins of efficient proton abstraction from carbon by a catalytic antibody
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作者：Erik W. Debler ; Shuichiro Ito ; Florian P. Seebeck 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2005
卷号：102
期号：14
页码：4984-4989
DOI：10.1073/pnas.0409207102
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Antibody 34E4 catalyzes the conversion of benzisoxazoles to salicylonitriles with high rates and multiple turnovers. The crystal structure of its complex with the benzimidazolium hapten at 2.5-A resolution shows that a combination of hydrogen bonding, {pi} stacking, and van der Waals interactions is exploited to position both the base, GluH50, and the substrate for efficient proton transfer. Suboptimal placement of the catalytic carboxylate, as observed in the 2.8-A structure of the GluH50Asp variant, results in substantially reduced catalytic efficiency. In addition to imposing high positional order on the transition state, the antibody pocket provides a highly structured microenvironment for the reaction in which the carboxylate base is activated through partial desolvation, and the highly polarizable transition state is stabilized by dispersion interactions with the aromatic residue TrpL91 and solvation of the leaving group oxygen by external water. The enzyme-like efficiency of general base catalysis in this system directly reflects the original hapten design, in which a charged guanidinium moiety was strategically used to elicit an accurately positioned functional group in an appropriate reaction environment and suggests that even larger catalytic effects may be achievable by extending this approach to the induction of acid-base pairs capable of bifunctional catalysis.
关键词：crystal structure ; base catalysis ; proton transfer ; medium effects ; orientation effects