文章基本信息

标题：Orthogonal analysis of C/EBPβ targets in vivo during liver proliferation
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作者：Joshua R. Friedman ; Brian Larris ; Phillip P. Le 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2004
卷号：101
期号：35
页码：12986-12991
DOI：10.1073/pnas.0402875101
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：CCAAT enhancer-binding protein {beta} (C/EBP{beta}), a basic-leucine zipper transcription factor, is an important effector of signals in physiologic growth and cancer. The identification of direct C/EBP{beta} targets in vivo has been limited by functional compensation by other C/EBP family proteins and the low stringency of the consensus sequence. Here we use the combined power of expression profiling and high-throughput chromatin immunoprecipitation to identify direct and biologically relevant targets of C/EBP{beta}. We identified 25 potential C/EBP{beta} targets, of which 88% of those tested were confirmed as in vivo C/EBP{beta}-binding sites. Six of these genes also displayed differential expression in C/EBP{beta}-/- livers. Computational analysis revealed that bona fide C/EBP{beta} target genes can be distinguished by the presence of binding motifs for specific additional transcription factors in the vicinity of the C/EBP{beta} site. This approach is generally applicable to the discovery of direct, biologically relevant targets of mammalian transcription factors.