文章基本信息

标题：Inducing and modulating anisotropic DNA bends by pseudocomplementary peptide nucleic acids
本地全文：下载
作者：Heiko Kuhn ; Dmitry I. Cherny ; Vadim V. Demidov 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2004
卷号：101
期号：20
页码：7548-7553
DOI：10.1073/pnas.0308756101
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：DNA bending is significant for various DNA functions in the cell. Here, we demonstrate that pseudocomplementary peptide nucleic acids (pcPNAs) represent a class of versatile, sequence-specific DNA-bending agents. The occurrence of anisotropic DNA bends induced by pcPNAs is shown by gel electrophoretic phasing analysis. The magnitude of DNA bending is determined by circular permutation assay and by electron microscopy, with good agreement of calculated mean values between both methods. Binding of a pair of 10-meric pcPNAs to its target DNA sequence results in moderate DNA bending with a mean value of 40-45{degrees