文章基本信息

标题：Stochastic model of protein–protein interaction: Why signaling proteins need to be colocalized
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作者：Nizar N. Batada ; Larry A. Shepp ; David O. Siegmund 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2004
卷号：101
期号：17
页码：6445-6449
DOI：10.1073/pnas.0401314101
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Colocalization of proteins that are part of the same signal transduction pathway via compartmentalization, scaffold, or anchor proteins is an essential aspect of the signal transduction system in eukaryotic cells. If interaction must occur via free diffusion, then the spatial separation between the sources of the two interacting proteins and their degradation rates become primary determinants of the time required for interaction. To understand the role of such colocalization, we create a mathematical model of the diffusion based protein-protein interaction process. We assume that mRNAs, which serve as the sources of these proteins, are located at different positions in the cytoplasm. For large cells such as Drosophila oocytes we show that if the source mRNAs were at random locations in the cell rather than colocalized, the average rate of interactions would be extremely small, which suggests that localization is needed to facilitate protein interactions and not just to prevent cross-talk between different signaling modules.
关键词：protein diffusion ; protein mobility ; intracellular reaction ; protein localization