文章基本信息

标题：Canonical pathway of nuclear factor κB activation selectively regulates proinflammatory and prothrombotic responses in human atherosclerosis
本地全文：下载
作者：Claudia Monaco ; Evangelos Andreakos ; Serafim Kiriakidis 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2004
卷号：101
期号：15
页码：5634-5639
DOI：10.1073/pnas.0401060101
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Nuclear factor {kappa}B (NF-{kappa}B) activation has been observed in human atherosclerotic plaques and is enhanced in unstable coronary plaques, but whether such activation has a protective or pathophysiological role remains to be determined. We addressed this question by developing a short-term culture system of cells isolated from human atherosclerotic tissue, allowing efficient gene transfer to directly investigate signaling pathways in human atherosclerosis. We found that NF-{kappa}B is activated in these cells and that this activity involves p65, p50, and c-Rel but not p52 or RelB. This NF-{kappa}B activation can be blocked by overexpression of I{kappa}B{alpha} or dominant-negative I{kappa}B kinase (IKK)-2 but not dominant-negative IKK-1 or NF-{kappa}B-inducing kinase, resulting in selective inhibition of inflammatory cytokines (tumor necrosis factor {alpha