文章基本信息

标题：A regulator that inhibits transcription by targeting an intersubunit interaction of the RNA polymerase holoenzyme
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作者：B. D. Gregory ; B. E. Nickels ; S. J. Garrity 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2004
卷号：101
期号：13
页码：4554-4559
DOI：10.1073/pnas.0400923101
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The structures of the bacterial RNA polymerase holoenzyme have provided detailed information about the intersubunit interactions within the holoenzyme. Functional analysis indicates that one of these is critical in enabling the holoenzyme to recognize the major class of bacterial promoters. It has been suggested that this interaction, involving the flap domain of the {beta} subunit and conserved region 4 of the {sigma} subunit, is a potential target for regulation. Here we provide genetic and biochemical evidence that the {sigma} region 4/{beta}-flap interaction is targeted by the transcription factor AsiA. Specifically, we show that AsiA competes directly with the {beta}-flap for binding to {sigma} region 4, thereby inhibiting transcription initiation by disrupting the {sigma} region 4/{beta}-flap interaction.