文章基本信息

标题：Yeast genome-wide drug-induced haploinsufficiency screen to determine drug mode of action
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作者：Kristin Baetz ; Lianne McHardy ; Ken Gable 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2004
卷号：101
期号：13
页码：4525-4530
DOI：10.1073/pnas.0307122101
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Methods to systematically test drugs against all possible proteins in a cell are needed to identify the targets underlying their therapeutic action and unwanted effects. Here, we show that a genome-wide drug-induced haploinsufficiency screen by using yeast can reveal drug mode of action in yeast and can be used to predict drug mode of action in human cells. We demonstrate that dihydromotuporamine C, a compound in preclinical development that inhibits angiogenesis and metastasis by an unknown mechanism, targets sphingolipid metabolism. The systematic, unbiased and genome-wide nature of this technique makes it attractive as a general approach to identify cellular pathways affected by drugs.