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标题：The role of RNA polymerase σ subunit in promoter-independent initiation of transcription
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作者：Nikolay Zenkin ; Konstantin Severinov
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2004
卷号：101
期号：13
页码：4396-4400
DOI：10.1073/pnas.0400886101
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：In bacteria, initiation of transcription depends on the RNA polymerase {sigma} subunit, which brings catalytically proficient RNA polymerase core to promoters by binding to specific DNA elements located upstream of the transcription start point. Here, we study {sigma}-dependent synthesis of a transcript that is used to prime replication of the single-stranded genome of bacteriophage M13. We show that, in this system, {sigma} plays no role in DNA recognition, which is accomplished solely through RNA polymerase core interaction with DNA downstream of the transcription start point. However, {sigma} is required for full-sized transcript synthesis by allowing RNA polymerase core to escape into productive elongation. RNA polymerase {sigma} may play a similar role during replication primer synthesis in other bacterial mobile elements whose life cycle involves a single-stranded DNA stage.