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标题：Human CD34+ cells differentiate into microglia and express recombinant therapeutic protein
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作者：Muriel Asheuer ; Françoise Pflumio ; Sonia Benhamida 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2004
卷号：101
期号：10
页码：3557-3562
DOI：10.1073/pnas.0306431101
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：In rodents, bone marrow-derived cells enter the brain during adult life. Allogeneic bone marrow transplantation is used to treat genetic CNS diseases, but the fate of human bone marrow and CD34+ cells within the brain remains to be elucidated. The present study demonstrates that cells derived from human CD34+ cells, isolated from either cord blood or peripheral blood, migrate into the brain after infusion into nonobese diabetic/severe combined immunodeficient mice. Both types of CD34+-derived cells differentiate into perivascular and ramified microglia. The lentiviral transfer of genes into CD34+ cells before infusion does not modify the differentiation of human CD34+ cells into microglia, allowing new transgenic proteins to be expressed in these cells. The transplantation of CD34+ cells could thus be used for the treatment of CNS diseases.