文章基本信息

标题：Identification of many microRNAs that copurify with polyribosomes in mammalian neurons
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作者：John Kim ; Anna Krichevsky ; Yonatan Grad 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2004
卷号：101
期号：1
页码：360-365
DOI：10.1073/pnas.2333854100
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Localized translation in mammalian dendrites may play a role in synaptic plasticity and contribute to the molecular basis for learning and memory. The regulatory mechanisms that control localized translation in neurons are not well understood. We propose a role for microRNAs (miRNAs), a class of noncoding RNAs, as mediators of neuronal translational regulation. We have identified 86 miRNAs expressed in mammalian neurons, of which 40 have not previously been reported. A subset of these miRNAs exhibits temporally regulated expression in cortical cultures. Moreover, all of the miRNAs that were tested cofractionate with polyribosomes, the sites of active translation. These findings indicate that a large, diverse population of miRNAs may function to regulate translation in mammalian neurons.
关键词：translational control ; noncoding RNA ; messenger ribonucleoprotein complex (mRNP)