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  • 标题:The two rotor components of yeast mitochondrial ATP synthase are mechanically coupled by subunit δ
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  • 作者:Stéphane Duvezin-Caubet ; Matthieu Caron ; Marie-France Giraud
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:2003
  • 卷号:100
  • 期号:23
  • 页码:13235-13240
  • DOI:10.1073/pnas.2135169100
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:The mitochondrial ATP synthase is made of a membrane-integrated F0 component that forms a proton-permeable pore through the inner membrane and a globular peripheral F1 domain where ATP is synthesized. The catalytic mechanism is thought to involve the rotation of a 10-12 c subunit ring in the F0 together with the {gamma} subunit of F1. An important and not yet resolved question is to define precisely how the {gamma} subunit is connected with the c-ring. In this study, using a doxycycline-regulatable expression system, we provide direct evidence that the rest of the enzyme can assemble without the {delta} subunit of F1, and we show that {delta}-less mitochondria are uncoupled because of an F0-mediated proton leak. Based on these observations, and taking into account high-resolution structural models, we propose that subunit {delta} plays a key role in the mechanical coupling of the c-ring to subunit {gamma}.
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