文章基本信息

标题：A specific endoribonuclease, RNase P, affects gene expression of polycistronic operon mRNAs
本地全文：下载
作者：Yong Li ; Sidney Altman
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2003
卷号：100
期号：23
页码：13213-13218
DOI：10.1073/pnas.2235589100
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The rnpA mutation, A49, in Escherichia coli reduces the level of RNase P at 43{degrees}C because of a temperature-sensitive mutation in C5 protein, the protein subunit of the enzyme. Microarray analysis reveals the expression of several noncoding intergenic regions that are increased at 43{degrees}C compared with 30{degrees}C. These regions are substrates for RNase P, and they are cleaved less efficiently than, for example, tRNA precursors. An analysis of the tna, secG, rbs, and his operons, all of which contain RNase P cleavage sites, indicates that RNase P affects gene expression for regions downstream of its cleavage sites.