文章基本信息

标题：Principles of cell-free genetic circuit assembly
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作者：Vincent Noireaux ; Roy Bar-Ziv ; Albert Libchaber 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2003
卷号：100
期号：22
页码：12672-12677
DOI：10.1073/pnas.2135496100
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Cell-free genetic circuit elements were constructed in a transcription-translation extract. We engineered transcriptional activation and repression cascades, in which the protein product of each stage is the input required to drive or block the following stage. Although we can find regions of linear response for single stages, cascading to subsequent stages requires working in nonlinear regimes. Substantial time delays and dramatic decreases in output production are incurred with each additional stage because of a bottleneck at the translation machinery. Faster turnover of RNA message can relieve competition between genes and stabilize output against variations in input and parameters.