文章基本信息

标题：Single-molecule detection of DNA hybridization
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作者：Mukta Singh-Zocchi ; Sanhita Dixit ; Vassili Ivanov 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2003
卷号：100
期号：13
页码：7605-7610
DOI：10.1073/pnas.1337215100
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We demonstrate the detection of nanometer-scale conformational changes of single DNA oligomers through a micromechanical technique. The quantity monitored is the displacement of a micrometer-size bead tethered to a surface by the probe molecule undergoing the conformational change. This technique allows probing of conformational changes within distances beyond the range of fluorescence resonance energy transfer. We apply the method to detect single hybridization events of label-free target oligomers. Hybridization of the target is detected through the conformational change of the probe.