文章基本信息

标题：Protein structure change studied by hydrogen-deuterium exchange, functional labeling, and mass spectrometry
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作者：Joan J. Englander ; Charyl Del Mar ; Will Li 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2003
卷号：100
期号：12
页码：7057-7062
DOI：10.1073/pnas.1232301100
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：An automated high-throughput, high-resolution deuterium exchange HPLC-MS method (DXMS) was used to extend previous hydrogen exchange studies on the position and energetic role of regulatory structure changes in hemoglobin. The results match earlier highly accurate but much more limited tritium exchange results, extend the analysis to the entire sequence of both hemoglobin subunits, and identify some energetically important changes. Allosterically sensitive amide hydrogens located at near amino acid resolution help to confirm the reality of local unfolding reactions and their use to evaluate resolved structure changes in terms of allosteric free energy.