首页    期刊浏览 2024年12月12日 星期四
登录注册

文章基本信息

  • 标题:Reverse engineering gene networks: Integrating genetic perturbations with dynamical modeling
  • 本地全文:下载
  • 作者:Jesper Tegnér ; M. K. Stephen Yeung ; Jeff Hasty
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:2003
  • 卷号:100
  • 期号:10
  • 页码:5944-5949
  • DOI:10.1073/pnas.0933416100
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:While the fundamental building blocks of biology are being tabulated by the various genome projects, microarray technology is setting the stage for the task of deducing the connectivity of large-scale gene networks. We show how the perturbation of carefully chosen genes in a microarray experiment can be used in conjunction with a reverse engineering algorithm to reveal the architecture of an underlying gene regulatory network. Our iterative scheme identifies the network topology by analyzing the steady-state changes in gene expression resulting from the systematic perturbation of a particular node in the network. We highlight the validity of our reverse engineering approach through the successful deduction of the topology of a linear in numero gene network and a recently reported model for the segmentation polarity network in Drosophila melanogaster. Our method may prove useful in identifying and validating specific drug targets and in deconvolving the effects of chemical compounds.
国家哲学社会科学文献中心版权所有