文章基本信息

标题：Mechanical disruption of individual nucleosomes reveals a reversible multistage release of DNA
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作者：Brent D. Brower-Toland ; Corey L. Smith ; Richard C. Yeh 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2002
卷号：99
期号：4
页码：1960-1965
DOI：10.1073/pnas.022638399
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The dynamic structure of individual nucleosomes was examined by stretching nucleosomal arrays with a feedback-enhanced optical trap. Forced disassembly of each nucleosome occurred in three stages. Analysis of the data using a simple worm-like chain model yields 76 bp of DNA released from the histone core at low stretching force. Subsequently, 80 bp are released at higher forces in two stages: full extension of DNA with histones bound, followed by detachment of histones. When arrays were relaxed before the dissociated state was reached, nucleosomes were able to reassemble and to repeat the disassembly process. The kinetic parameters for nucleosome disassembly also have been determined.