文章基本信息

标题：Eukaryotic-type elongator tRNAMet of Trypanosoma brucei becomes formylated after import into mitochondria
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作者：Timothy H. P. Tan ; Natacha Bochud-Allemann ; Elke K. Horn 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2002
卷号：99
期号：3
页码：1152-1157
DOI：10.1073/pnas.022522299
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The mitochondrion of Trypanosoma brucei lacks tRNA genes. Its translation system therefore depends on the import of cytosolic, nucleus-encoded tRNAs. Thus, most trypanosomal tRNAs function in both the cytosol and the mitochondrion, and all are of the eukaryotic type. This is also the case for the elongator tRNAMet, whereas the only other trypanosomal tRNAMet, the eukaryotic initiator, is found exclusively in the cytosol. Unlike their cytosolic counterparts, organellar initiator tRNAsMet carry a formylated methionine. This raises the question of how initiation of translation works in trypanosomal mitochondria, where only elongator tRNAMet is found. Using in organello charging and formylation assays, we show that unexpectedly a fraction of elongator tRNAMet becomes formylated after import into mitochondria. Furthermore, in vitro experiments with mitochondrial extracts demonstrate that only the trypanosomal elongator and not the initiator tRNAMet is recognized by the formylation activity. Finally, RNA interference assays identify the gene encoding the trypanosomal formylase activity. Whereas the predicted protein is homologous to prokaryotic and mitochondrial methionyl-tRNAMet formyltransferases, it has about twice the mass of any of these proteins.
关键词：mitochondrial translation‖initiator tRNA^Met‖formylation‖mitochondrial tRNA import‖methionyl-tRNA^Met formyltransferase