文章基本信息

标题：Noninvasive imaging of protein–protein interactions in living subjects by using reporter protein complementation and reconstitution strategies
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作者：R. Paulmurugan ; Y. Umezawa ; S. S. Gambhir 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2002
卷号：99
期号：24
页码：15608-15613
DOI：10.1073/pnas.242594299
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：In this study we have developed bioluminescence-imaging strategies to noninvasively and quantitatively image protein--protein interactions in living mice by using a cooled charge-coupled device camera and split reporter technology. We validate both complementation and intein-mediated reconstitution of split firefly luciferase proteins driven by the interaction of two strongly interacting proteins, MyoD and Id. We use transient transfection of cells and image MyoD-Id interaction after induction of gene expression in cell culture and in cells implanted into living mice. Techniques to study protein-protein interactions in living subjects will allow the study of cellular networks, including signal transduction pathways, as well as development and optimization of pharmaceuticals for modulating protein-protein interactions.