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标题：Determinants of the cellular specificity of acetaminophen as an inhibitor of prostaglandin H2 synthases
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作者：Olivier Boutaud ; David M. Aronoff ; Jacob H. Richardson 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2002
卷号：99
期号：10
页码：7130-7135
DOI：10.1073/pnas.102588199
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Acetaminophen has antipyretic and analgesic properties yet differs from the nonsteroidal antiinflammatory drugs and inhibitors of prostaglandin H synthase (PGHS)-2 by exhibiting little effect on platelets or inflammation. We find parallel selectivity at a cellular level; acetaminophen inhibits PGHS activity with an IC50 of 4.3 {micro}M in interleukin (IL)-1-stimulated human umbilical vein endothelial cells, in contrast with an IC50 of 1,870 {micro}M for the platelet, with 2 {micro}M arachidonic acid as substrate. This difference is not caused by isoform selectivity, because acetaminophen inhibits purified ovine PGHS-1 and murine recombinant PGHS-2 equally. We explored the hypothesis that this difference in cellular responsiveness results from antagonism of the reductant action of acetaminophen on the PGHSs by cellular peroxides. Increasing the peroxide product of the PGHS-cyclooxygenase, prostaglandin G2 (PGG2), by elevating the concentration of either enzyme or substrate reverses the inhibitory action of acetaminophen, as does the addition of PGG2 itself. 12-Hydroperoxyeicosatetraenoic acid (0.3 {micro}M), a major product of the platelet, completely reverses the action of acetaminophen on PGHS-1. Inhibition of PGHS activity by acetaminophen in human umbilical vein endothelial cells is abrogated by t-butyl hydroperoxide. Together these findings support the hypothesis that the clinical action of acetaminophen is mediated by inhibition of PGHS activity, and that hydroperoxide concentration contributes to its cellular selectivity.