文章基本信息

标题：Activation by GDNF of a transcriptional program repressing neurite growth in dorsal root ganglia
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作者：Sten Linnarsson ; Åsa Mikaels ; Christel Baudet 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2001
卷号：98
期号：25
页码：14681-14686
DOI：10.1073/pnas.251548898
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Glial cell line-derived neurotrophic factor (GDNF) promotes the survival of postnatal--but not embryonic--mouse dorsal root ganglion cells in vitro, despite the fact that its receptors are expressed at both ages. To understand this difference, we have performed an oligonucleotide microarray experiment. We found that several hundred genes were regulated between embryonic and postnatal stages, and that several important classes of genes were differentially regulated by GDNF treatment, including genes related to translation and to phenotypic specification and maturation. Interestingly, a set of genes related to cell adhesion, cytoskeleton and cellular morphology were consistently down-regulated by GDNF, suggesting a previously uncharacterized role for GDNF in repressing neurite growth and/or branching. This nuclear program initiated by GDNF was functionally confirmed in cultures of embryonic wild-type neurons sustained with nerve growth factor and in bax-/- neurons that survive in the absence of trophic support.
关键词：neurotrophic‖microarray