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标题：Pause and rotation of F1-ATPase during catalysis
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作者：Yoko Hirono-Hara ; Hiroyuki Noji ; Masaya Nishiura 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2001
卷号：98
期号：24
页码：13649-13654
DOI：10.1073/pnas.241365698
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：F1-ATPase is a rotary motor enzyme in which a single ATP molecule drives a 120{degrees} rotation of the central {gamma} subunit relative to the surrounding 3{beta}3 ring. Here, we show that the rotation of F1-ATPase spontaneously lapses into long ({approx}30 s) pauses during steady-state catalysis. The effects of ADP-Mg and mutation on the pauses, as well as kinetic comparison with bulk-phase catalysis, strongly indicate that the paused enzyme corresponds to the inactive state of F1-ATPase previously known as the ADP-Mg inhibited form in which F1-ATPase fails to release ADP-Mg from catalytic sites. The pausing position of the {gamma} subunit deviates from the ATP-waiting position and is most likely the recently found intermediate 90{degrees} position.