文章基本信息

标题：A regulatory protein that interferes with activator-stimulated transcription in bacteria
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作者：Shunji Nakano ; Michiko M. Nakano ; Ying Zhang 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2003
卷号：100
期号：7
页码：4233-4238
DOI：10.1073/pnas.0637648100
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Transcriptional activator proteins in bacteria often operate by interaction with the C-terminal domain of the -subunit of RNA polymerase (RNAP). Here we report the discovery of an "anti-" factor Spx in Bacillus subtilis that blocks transcriptional activation by binding to the -C-terminal domain, thereby interfering with the capacity of RNAP to respond to certain activator proteins. Spx disrupts complex formation between the activator proteins ResD and ComA and promoter-bound RNAP, and it does so by direct interaction with the -subunit. ResD- and ComA-stimulated transcription requires the proteolytic elimination of Spx by the ATP-dependent protease ClpXP. Spx represents a class of transcriptional regulators that inhibit activator-stimulated transcription by interaction with .
关键词：Spx‖RNA polymerase‖α-subunit‖ Bacillus subtilis ‖transcriptional activation