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标题：Long-term depression of climbing fiber-evoked calcium transients in Purkinje cell dendrites
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作者：John T. Weber ; Chris I. De Zeeuw ; David J. Linden 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2003
卷号：100
期号：5
页码：2878-2883
DOI：10.1073/pnas.0536420100
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：In recent years much has been learned about the molecular requirements for inducing long-term synaptic depression (LTD) in various brain regions. However, very little is known about the consequences of LTD induction for subsequent signaling events in postsynaptic neurons. We have addressed this issue by examining homosynaptic LTD at the cerebellar climbing fiber (CF)-Purkinje cell (PC) synapse. This synapse is built for reliable and massive excitation: Activation of a single axon produces an unusually large -amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor-mediated synaptic current, the depolarization of which drives a regenerative complex spike producing a large, widespread Ca2+ transient in PC dendrites. Here we test whether CF LTD has an impact on dendritic, complex spike-evoked Ca2+ signals by simultaneously performing long-term recordings of complex spikes and microfluorimetric Ca2+ measurements in PC dendrites in rat cerebellar slices. Our data show that LTD of the CF excitatory postsynaptic current produces a reduction in both slow components of the complex spike waveform and complex spike-evoked dendritic Ca2+ transients. This LTD of dendritic Ca2+ signals may provide a neuroprotective mechanism and/or constitute "heterosynaptic metaplasticity" by reducing the probability for subsequent induction of those forms of use-dependent plasticity, which require CF-evoked Ca2+ signals such as parallel fiber-PC LTD and interneuron-PC LTP.