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标题：Microbe forensics: Oxygen and hydrogen stable isotope ratios in Bacillus subtilis cells and spores
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作者：Helen W. Kreuzer-Martin ; Michael J. Lott ; Janet Dorigan 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2003
卷号：100
期号：3
页码：815-819
DOI：10.1073/pnas.252747799
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Bacillus subtilis, a Gram-positive, endospore-forming soil bacterium, was grown in media made with water of varying oxygen ({delta}18O) and hydrogen ({delta}D) stable isotope ratios. Logarithmically growing cells and spores were each harvested from the cultures and their {delta}18O and {delta}D values determined. Oxygen and hydrogen stable isotope ratios of organic matter were linearly related with those of the media water. We used the relationships determined in these experiments to calculate the effective whole-cell fractionation factors between water and organic matter for B. subtilis. We then predicted the {delta}18O and {delta}D values of spores produced in nutritionally identical media and local water sources for five different locations around the United States. Each of the measured {delta}18O and {delta}D values of the spores matched the predicted values within a 95% confidence interval, indicating that stable isotope ratio analyses may be a powerful tool for tracing the geographic point-of-origin for microbial products.