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标题：A role for estrogen receptor β in the regulation of growth of the ventral prostate
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作者：Zhang Weihua ; Sari Mäkelä ; Leif C. Andersson 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2001
卷号：98
期号：11
页码：6330-6335
DOI：10.1073/pnas.111150898
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：In normal rats and mice, immunostaining with specific antibodies revealed that nuclei of most prostatic epithelial cells harbor estrogen receptor {beta} (ER{beta}). In rat ventral prostate, 530- and 549-aa isoforms of the receptor were identified. These sediment in the 4S region of low-salt sucrose gradients, indicating that prostatic ER{beta} does not contain the same protein chaperones that are associated with ER. Estradiol (E2) binding and ER{beta} immunoreactivity coincide on the gradient, with no indication of ER. In prostates from mice in which the ER{beta} gene has been inactivated (BERKO), androgen receptor (AR) levels are elevated, and the tissue contains multiple hyperplastic foci. Most epithelial cells express the proliferation antigen Ki-67. In contrast, prostatic epithelium from wild-type littermates is single layered with no hyperplasia, and very few cells express Ki-67. Rat ventral prostate contains an estrogenic component, which comigrates on HPLC with the testosterone metabolite 5-androstane-3{beta