文章基本信息

标题：A type IB topoisomerase with DNA repair activities
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作者：Galina I. Belova ; Rajendra Prasad ; Sergei A. Kozyavkin 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2001
卷号：98
期号：11
页码：6015-6020
DOI：10.1073/pnas.111040498
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Previously we have characterized type IB DNA topoisomerase V (topo V) in the hyperthermophile Methanopyrus kandleri. The enzyme has a powerful topoisomerase activity and is abundant in M. kandleri. Here we report two characterizations of topo V. First, we found that its N-terminal domain has sequence homology with both eukaryotic type IB topoisomerases and the integrase family of tyrosine recombinases. The C-terminal part of the sequence includes 12 repeats, each repeat consisting of two similar but distinct helix-hairpin-helix motifs; the same arrangement is seen in recombination protein RuvA and mammalian DNA polymerase {beta}. Second, on the basis of sequence homology between topo V and polymerase {beta