文章基本信息

标题：The molecular basis of vancomycin resistance in clinically relevant Enterococci: Crystal structure of d-alanyl-d-lactate ligase (VanA)
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作者：David I. Roper ; Trevor Huyton ; Alexei Vagin 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2000
卷号：97
期号：16
页码：8921-8925
DOI：10.1073/pnas.150116497
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：D-alanine-D-lactate ligase from Enterococcus faecium BM4147 is directly responsible for the biosynthesis of alternate cell-wall precursors in bacteria, which are resistant to the glycopeptide antibiotic vancomycin. The crystal structure has been determined with data extending to 2.5-A resolution. This structure shows that the active site has unexpected interactions and is distinct from previous models for D-alanyl-D-lactate ligase mechanistic studies. It appears that the preference of the enzyme for lactate as a ligand over D-alanine could be mediated by electrostatic effects and/or a hydrogen-bonding network, which principally involve His-244. The structure of D-alanyl-D-lactate ligase provides a revised interpretation of the molecular events that lead to vancomycin resistance.
关键词：x-ray crystal structure