文章基本信息

标题：Biosynthesis of terpenoids: 4-Diphosphocytidyl-2-C-methyl-d-erythritol kinase from tomato
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作者：Markus Fischer ; Wolfgang Eisenreich ; Christoph A. Schuhr 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2000
卷号：97
期号：15
页码：8251-8256
DOI：10.1073/pnas.140209197
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The putative catalytic domain (residues 81-401) of a predicted tomato protein with similarity to 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase of Escherichia coli was expressed in a recombinant E. coli strain. The protein was purified to homogeneity and was shown to catalyze the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2-C-methyl-D-erythritol at a rate of 33 {micro}mol*mg-1*min-1. The structure of the reaction product, 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate, was established by NMR spectroscopy. Divalent metal ions, preferably Mg2+, are required for activity. Neither the tomato enzyme nor the E. coli ortholog catalyzes the phosphorylation of isopentenyl monophosphate.