文章基本信息

标题：Apoptin induces apoptosis in human transformed and malignant cells but not in normal cells
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作者：A. A. A. M. Danen-Van Oorschot ; D. F. Fischer ; J. M. Grimbergen 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1997
卷号：94
期号：11
页码：5843-5847
DOI：10.1073/pnas.94.11.5843
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The chicken anemia virus protein apoptin induces a p53-independent, Bcl-2-insensitive type of apoptosis in various human tumor cells. Here, we show that, in vitro, apoptin fails to induce programmed cell death in normal lymphoid, dermal, epidermal, endothelial, and smooth-muscle cells. However, when normal cells are transformed they become susceptible to apoptosis by apoptin. Long-term expression of apoptin in normal human fibroblasts revealed that apoptin has no toxic or transforming activity in these cells. In normal cells, apoptin was found predominantly in the cytoplasm, whereas in transformed and malignant cells it was located in the nucleus, suggesting that the localization of apoptin is related to its activity. These properties make apoptin a potential agent for the treatment of a large number of tumors, also those lacking p53 and/or overexpressing Bcl-2.
关键词：programmed cell death ; tumor cells ; antitumor agent