文章基本信息

标题：A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications
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作者：Hao Chang ; Mingshu Zhang ; Wei Ji 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2012
卷号：109
期号：12
页码：4455-4460
DOI：10.1073/pnas.1113770109
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Reversibly switchable fluorescent proteins (RSFPs) have attracted widespread interest for emerging techniques including repeated tracking of protein behavior and superresolution microscopy. Among the limited number of RSFPs available, only Dronpa is widely employed for most cell biology applications due to its monomeric and other favorable photochemical properties. Here we developed a series of monomeric green RSFPs with beneficial optical characteristics such as high photon output per switch, high photostability, a broad range of switching rate, and pH-dependence, which make them potentially useful for various applications. One member of this series, mGeos-M, exhibits the highest photon budget and localization precision potential among all green RSFPs. We propose mGeos-M as a candidate to replace Dronpa for applications such as dynamic tracking, dual-color superresolution imaging, and optical lock-in detection.
关键词：(F)PALM/STORM ; mEos2 ; mGeos ; superresolution microscopy