文章基本信息

标题：Genetic code translation displays a linear trade-off between efficiency and accuracy of tRNA selection
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作者：Magnus Johansson ; Jingji Zhang ; Måns Ehrenberg 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2012
卷号：109
期号：1
页码：131-136
DOI：10.1073/pnas.1116480109
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Rapid and accurate translation of the genetic code into protein is fundamental to life. Yet due to lack of a suitable assay, little is known about the accuracy-determining parameters and their correlation with translational speed. Here, we develop such an assay, based on Mg2+ concentration changes, to determine maximal accuracy limits for a complete set of single-mismatch codon-anticodon interactions. We found a simple, linear trade-off between efficiency of cognate codon reading and accuracy of tRNA selection. The maximal accuracy was highest for the second codon position and lowest for the third. The results rationalize the existence of proofreading in code reading and have implications for the understanding of tRNA modifications, as well as of translation error-modulating ribosomal mutations and antibiotics. Finally, the results bridge the gap between in vivo and in vitro translation and allow us to calibrate our test tube conditions to represent the environment inside the living cell.
关键词：fidelity ; rate-accuracy trade-off ; ribosome ; protein synthesis ; elongation