文章基本信息

标题：Converting structural information into an allosteric-energy-based picture for elongation factor Tu activation by the ribosome
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作者：Andrew J. Adamczyk ; Arieh Warshel
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2011
卷号：108
期号：24
页码：9827-9832
DOI：10.1073/pnas.1105714108
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The crucial process of aminoacyl-tRNA delivery to the ribosome is energized by the GTPase reaction of the elongation factor Tu (EF-Tu). Advances in the elucidation of the structure of the EF-Tu/ribosome complex provide the rare opportunity of gaining a detailed understanding of the activation process of this system. Here, we use quantitative simulation approaches and reproduce the energetics of the GTPase reaction of EF-Tu with and without the ribosome and with several key mutants. Our study provides a novel insight into the activation process. It is found that the critical H84 residue is not likely to behave as a general base but rather contributes to an allosteric effect, which includes a major transition state stabilization by the electrostatic effect of the P loop and other regions of the protein. Our findings have general relevance to GTPase activation, including the processes that control signal transduction.
关键词：enzymatic catalysis ; preorganization ; allostery