文章基本信息

标题：β Ca2+/CaM-dependent kinase type II triggers upregulation of GluA1 to coordinate adaptation to synaptic inactivity in hippocampal neurons
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作者：Rachel D. Groth ; Maria Lindskog ; Tara C. Thiagarajan 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2011
卷号：108
期号：2
页码：828-833
DOI：10.1073/pnas.1018022108
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Prolonged AMPA-receptor blockade in hippocampal neuron cultures leads to both an increased expression of GluA1 postsynaptically and an increase in vesicle pool size and turnover rate presynaptically, adaptive changes that extend beyond simple synaptic scaling. As a molecular correlate, expression of the {beta} Ca2+/CaM-dependent kinase type II ({beta}CaMKII) is increased in response to synaptic inactivity. Here we set out to clarify the role of {beta}CaMKII in the various manifestations of adaptation. Knockdown of {beta}CaMKII by lentiviral-mediated expression of shRNA prevented the synaptic inactivity-induced increase in GluA1, as did treatment with the CaM kinase inhibitor KN-93, but not the inactive analog KN-92. These results demonstrate that, spurred by AMPA-receptor blockade, up-regulation of {beta}CaMKII promotes increased GluA1 expression. Indeed, transfection of {beta}CaMKII, but not a kinase-dead mutant, increased GluA1 expression on dendrites and elevated vesicle turnover (Syt-Ab uptake), mimicking the effect of synaptic inactivity on both sides of the synapse. In cells with elevated {beta}CaMKII, relief of synaptic-activity blockade uncovered an increase in the frequency of miniature excitatory postsynaptic currents that could be rapidly and fully suppressed by PhTx blockade of GluA1 receptors. This increased mini frequency involved a genuine presynaptic enhancement, not merely an increased abundance of synapses. This finding suggests that Ca2+ flux through GluA1 receptors may trigger the acute release of a retrograde messenger. Taken together, our results indicate that synaptic inactivity-induced increases in {beta}CaMKII expression set in motion a series of events that culminate in coordinated pre- and postsynaptic adaptations in synaptic transmission.
关键词：α Ca²⁺/CaM-dependent kinase type II ; homeostasis ; retrograde signaling ; synaptic coordination