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标题：Unitary response of mouse olfactory receptor neurons
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作者：Yair Ben-Chaim ; Melody M. Cheng ; King-Wai Yau 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2011
卷号：108
期号：2
页码：822-827
DOI：10.1073/pnas.1017983108
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The sense of smell begins with odorant molecules binding to membrane receptors on the cilia of olfactory receptor neurons (ORNs), thereby activating a G protein, Golf, and the downstream effector enzyme, an adenylyl cyclase (ACIII). Recently, we have found in amphibian ORNs that an odorant-binding event has a low probability of activating sensory transduction at all; even when successful, the resulting unitary response apparently involves a single active Golf-ACIII molecular complex. This low amplification is in contrast to rod phototransduction in vision, the best-quantified G-protein signaling pathway, where each photoisomerized rhodopsin molecule is well known to produce substantial amplification by activating many G-protein, and hence effector-enzyme, molecules. We have now carried out similar experiments on mouse ORNs, which offer, additionally, the advantage of genetics. Indeed, we found the same low probability of transduction, based on the unitary olfactory response having a fairly constant amplitude and similar kinetics across different odorants and randomly encountered ORNs. Also, consistent with our picture, the unitary response of Golf+/- ORNs was similar to WT in amplitude, although their Golf-protein expression was only half of normal. Finally, from the action potential firing, we estimated that [≤]19 odorant-binding events successfully triggering transduction in a WT mouse ORN will lead to signaling to the brain.
关键词：mammal ; olfaction ; olfactory transduction