文章基本信息

标题：Direct visualization reveals dynamics of a transient intermediate during protein assembly
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作者：Xin Zhang ; Vinh Q. Lam ; Yun Mou 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2011
卷号：108
期号：16
页码：6450-6455
DOI：10.1073/pnas.1019051108
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Interactions between proteins underlie numerous biological functions. Theoretical work suggests that protein interactions initiate with formation of transient intermediates that subsequently relax to specific, stable complexes. However, the nature and roles of these transient intermediates have remained elusive. Here, we characterized the global structure, dynamics, and stability of a transient, on-pathway intermediate during complex assembly between the Signal Recognition Particle (SRP) and its receptor. We show that this intermediate has overlapping but distinct interaction interfaces from that of the final complex, and it is stabilized by long-range electrostatic interactions. A wide distribution of conformations is explored by the intermediate; this distribution becomes more restricted in the final complex and is further regulated by the cargo of SRP. These results suggest a funnel-shaped energy landscape for protein interactions, and they provide a framework for understanding the role of transient intermediates in protein assembly and biological regulation.
关键词：EPR spectroscopy ; fluorescence spectroscopy ; molecular recognition ; protein targeting ; GTPases