文章基本信息

标题：Monosynaptic circuit tracing in vivo through Cre-dependent targeting and complementation of modified rabies virus
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作者：Nicholas R. Wall ; Ian R. Wickersham ; Ali Cetin 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2010
卷号：107
期号：50
页码：21848-21853
DOI：10.1073/pnas.1011756107
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We describe a powerful system for revealing the direct monosynaptic inputs to specific cell types in Cre-expressing transgenic mice through the use of Cre-dependent helper virus and a modified rabies virus. We generated helper viruses that target gene expression to Cre-expressing cells, allowing us to control initial rabies virus infection and subsequent monosynaptic retrograde spread. Investigators can use this system to elucidate the connections onto a desired cell type in a high-throughput manner, limited only by the availability of Cre mouse lines. This method allows for identification of circuits that would be extremely tedious or impossible to study with other methods and can be used to build subcircuit maps of inputs onto many different types of cells within the same brain region. Furthermore, by expressing various transgenes from the rabies genome, this system also has the potential to allow manipulation of targeted neuronal circuits without perturbing neighboring cells.
关键词：transsynaptic ; pseudotyped virus ; adeno-associated virus ; EnvA ; TVA