文章基本信息

标题：Cyclophilin 40 is required for microRNA activity in Arabidopsis
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作者：Michael R. Smith ; Matthew R. Willmann ; Gang Wu 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2009
卷号：106
期号：13
页码：5424-5429
DOI：10.1073/pnas.0812729106
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Loss-of-function mutations of SQUINT (SQN)--which encodes the Arabidopsis orthologue of cyclophilin 40 (CyP40)--cause the precocious expression of adult vegetative traits, an increase in carpel number, and produce abnormal spacing of flowers in the inflorescence. Here we show that the vegetative phenotype of sqn is attributable to the elevated expression of miR156-regulated members of the SPL family of transcription factors and provide evidence that this defect is a consequence of a reduction in the activity of ARGONAUTE1 (AGO1). Support for this latter conclusion was provided by the phenotypic similarity between hypomorphic alleles of AGO1 and null alleles of SQN and by the genetic interaction between sqn and these alleles. Our results suggest that AGO1, or an AGO1-interacting protein, is a major client of CyP40 and that miR156 and its targets play a central role in the regulation of vegetative phase change in Arabidopsis.
关键词：CyP40 ; HSP90 ; miR156 ; phase change ; immunophilin