文章基本信息

标题：Highly l and d enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection method
本地全文：下载
作者：Eugene Antipov ; Art E. Cho ; K. Dane Wittrup 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2008
卷号：105
期号：46
页码：17694-17699
DOI：10.1073/pnas.0809851105
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A highly efficient selection method for enhanced enzyme enantioselectivity based on yeast surface display and fluorescence-activated cell sorting (FACS) is developed and validated. Its application to horseradish peroxidase has resulted in enzyme variants up to 2 orders of magnitude selective toward either substrate enantiomer at will. These marked improvements in enantioselectivity are demonstrated for the surface-bound and soluble enzymes and rationalized by computational docking studies.
关键词：directed evolution ; enzyme design ; molecular modeling ; redox enzymes ; stereoselectivity