文章基本信息

标题：High-throughput sequencing allows the identification of binding molecules isolated from DNA-encoded chemical libraries
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作者：Luca Mannocci ; Yixin Zhang ; Jörg Scheuermann 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2008
卷号：105
期号：46
页码：17670-17675
DOI：10.1073/pnas.0805130105
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：DNA encoding facilitates the construction and screening of large chemical libraries. Here, we describe general strategies for the stepwise coupling of coding DNA fragments to nascent organic molecules throughout individual reaction steps as well as the first implementation of high-throughput sequencing for the identification and relative quantification of the library members. The methodology was exemplified in the construction of a DNA-encoded chemical library containing 4,000 compounds and in the discovery of binders to streptavidin, matrix metalloproteinase 3, and polyclonal human IgG.
关键词：drug discovery ; immunoglobulins ; streptavidin ; selection ; affinity chromatography