文章基本信息

标题：Concerted ATP-induced allosteric transitions in GroEL facilitate release of protein substrate domains in an all-or-none manner
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作者：Yakov Kipnis ; Niv Papo ; Gilad Haran 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2007
卷号：104
期号：9
页码：3119-3124
DOI：10.1073/pnas.0700070104
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The double-ring chaperonin GroEL mediates protein folding, in conjunction with its helper protein GroES, by undergoing ATP-induced conformational changes that are concerted within each heptameric ring. Here we have examined whether the concerted nature of these transitions is responsible for protein substrate release in an all-or-none manner. Two chimeric substrates were designed, each with two different reporter activities that were recovered after denaturation in GroES-dependent and independent fashions, respectively. The refolding of the chimeras was monitored in the presence of GroEL variants that undergo ATP-induced intraring conformational changes that are either sequential (F44W/D155A) or concerted (F44W). Our results show that release of a protein substrate from GroEL in a domain-by-domain fashion is favored when the intraring allosteric transitions of GroEL are sequential and not concerted.
关键词：allostery ; chaperonins ; cooperativity ; protein folding